Little is known about the cause of restless legs syndrome (RLS). In a study published online in Genome Research, researchers have demonstrated a common genetic variant associated with RLS alters expression of a critical brain gene during fetal development.
RLS is not caused by a single genetic defect, but rather is a complex disorder influenced by many genetic and environmental components, with age being the strongest risk factor. Previously, researchers identified genetic variants in RLS patients; however, how these variants, each of which only has a small effect, contributed to RLS was unclear.
In this new study, authors demonstrate how one of these variants may contribute to RLS. The RLS-associated variant is located in a non-coding region of the genome, and, when introduced into zebrafish and mouse embryos, led to decreased ability of the non-coding region to activate gene expression. Specifically, the authors observed this reduction in the future basal ganglia. “Here we have pinpointed down to an anatomical region for RLS,” says lead author of the study, Juliane Winkelmann, in a release.
Interestingly, the non-coding region seems only active during early brain development, suggesting that RLS, which is associated with aging, may have fetal origins. “Minor alterations in the developing forebrain during early embryonic development are probably leading to a predisposition to RLS,” Winkelmann says. “Later in life, during aging, and together with environmental factors, these may lead to the manifestation of the disease.”
The RLS-associated variant is located in an intron of MEIS1, a transcription factor involved in organ development and maintenance. The future basal ganglia, where the non-coding region is active, is also where MEIS1 is expressed, suggesting the non-coding region could be controlling MEIS1 expression. The risk variant binds more strongly to the transcriptional regulator CREB1, which may lead to the reduced MEIS1 expression. Furthermore, mice with reduced MEIS1 expression display hyperactivity, which resembles the human condition of RLS.
This study provides one of the first in-depth examinations of a genetic variant identified in a genome-wide association study, which examines many individuals for genetic variants that are linked to a trait. Although many variants are often reported in these studies, it has been difficult to understand how variants contribute to disease because they often lie in non-coding regions of the genome and have small effect sizes. “This work also reveals that combinatorial use of multiple approaches will be likely required to unravel the physiological causes of most human diseases,” co-author Jose Luis Gomez-Skarmeta says.